Bioanálisis de residuos de insecticidas con moscas Drosophila de alas vestigiales

Abstract

The method presented here is based on the use of adult vestigial-uinged Dro-Dro-sophila flies as bio-detectors of insecticidal residues in macerated products. sophila melanogaster Meig, vestigial-winged flies are raised on a "Imtox" formulated banana-agar medium Forty-eight bouts previons to the bio-assay all adult flies are removed from the culture medium, so only the neuly-emerged flies are used for exposure to the test material The vandomized testing samples are properly macerated with the aid of electric blenders and carefully protected from any possible contam ination Small amounts of alcohol and sometimes water may be added to the samples tested. When standards of comparison are needed the alcohol serves as the solvent for the known amounts of the insecticides being considered A number of the vestigial-winged Drosophilas anesthetized with CO, is placed into the testing cups Each analytical series includes the various testing samples, the standards, and the true check. Some products require slight modifications of the method described. The ppm. of insecticidal residues may be determined by comparing the mortalities obtained from the samples with the mortality curve of the standards.