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dc.contributor.authorRivera, C
dc.contributor.authorGámez, R.
dc.date.accessioned2022-09-14T17:29:32Z
dc.date.available2022-09-14T17:29:32Z
dc.date.issued1983-04
dc.identifier.urihttps://repositorio.catie.ac.cr/handle/11554/12006
dc.description.abstractAn enzyme-linked immunosorbent assay (EWA ) was adapted for the detection of maize rayado lino vims (REV) in its insect vector Dalbulus midis. Optimization of experimental conditions for the assay included the use of anti-REV immunoglobulin G (IgG) purified by ion exchange chromatography and the determination of critical concentrations of the enzyme-IgG conjugate, Tween 20 and bovine serum albumen. Under the experimental conditions described in this work, it was possible to detect viral antigens in individual leafhoppers at concentrations of less than 1.0 ng.es_ES
dc.format.extent8 páginases_ES
dc.language.isoeses_ES
dc.publisherInstituto Interamericano de Cooperación para la Agricultura (IICA)es_ES
dc.relation.ispartofTurrialba; Vol. 33, no. 2es_ES
dc.subjectELISAes_ES
dc.subjectDALBULUS MAIDISes_ES
dc.subjectVIRUS DE LAS PLANTASes_ES
dc.subjectZEA MAYSes_ES
dc.subjectELISAes_ES
dc.subjectDALBULUS MAIDISes_ES
dc.subjectPLANT VIRUSESes_ES
dc.subjectZEA MAYSes_ES
dc.subject.otherSede Centrales_ES
dc.titleAdaptación de una técnica de inmunosorbencia (ELISA) a la detección del virus del rayado fino del maíz en su insecto vectores_ES
dc.title.alternativeAdaptation of an immunosorbent assay (ELISA) for the detection of maize rayado fino virus in its insect vector.es_ES
dc.typeArtículoes_ES
dc.identifier.statusopenAccesses_ES


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