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Aislamiento de protoplastos del mesofilo del género Coffea
(Instituto Interamericano de Cooperación para la Agricultura (IICA), 1984-10)
Se describe un método rápido pare el aislamiento de protoplastos a partir de hojas de café, probándose diferentes enzimas en varias combinaciones. Es posible liberar abundante cantidad de protoplastos a partir de hojas ...
Somatic embryogenesis of Arabica coffee in temporary immersion culture: Advances, limitations, and perspectives for mass propagation of selected genotypes
(Plant Science, 2022-10)
Culture in temporary immersion systems (TIS) is a valuable tool for the semiautomation of high frequency somatic embryogenesis of coffee. This system allows the intermittent exposure of explants to liquid medium in cycles ...
SNP markers found in non‑coding regions can distinguish among low‑variant genotypes of Arabica and other coffee species
(Genetic Resources and Crop Evolution, 2022)
Development of efficient and scalable methods for molecular identification of Coffea spp. are necessary to accelerate studies related to the characterization of germplasm for both conservation or breeding purposes, and the ...